M.C. Thaller

Bachelor in Biotechnology (8 CC)

General Microbiology and Virology

MODULE I General Microbiology
Historical notes: the evolution of techniques and ideas.
Microbiological methods: cultivation, identification, taxonomy.
Cellular and molecular organization of prokaryotic and eukaryotic cells . The cell wall. The cytoplasmic membrane, transport and secretion systems. Cytoplasmic inclusions, outer envelopes. The organization and structure of the genome. Bacterial metabolism. Anabolism and catabolism; nutritional types and energy scavenging strategies: aerobic and anaerobic respiration, fermentation, oxygenic and anoxygenic photosynthesis. The environmental impact of microbial metabolism: basic knowledge about carbon and nitrogen cycles.
Microbial Growth: nutritional requirements, the struggle for food, nutrient transport and motility. Cell and population growth: Growth curve, abiotic factors, chemo- and other taxis. Sessile growth: the biofilms.
Bacteriophages.
Eucaryotics microorganisms: yeasts, molds and protozoans Genetics and genic expression regulations. Cell division, nucleoid replication and septum formation. Cell-cycle models. Mutations, lateral genetic transfer.Mobile DNA. Homologous and non- Homologous genetic recombination. Gene expression and regulation; Environment and microorganisms: microbes and biogeochemical cycles (carbon and nitrogen cycles), waste-waters treatment and compost production. Micro-organisms and other living beings: interactions between microorganisms, and microorganisms/Eucarya. Parasite/host interactions, virulence Antimicrobial strategies: sterilization, disinfection, antibiotics, action and resistance.
Basics of Immunology: Humoral and cell-mediated immunity. Innate and acquired immunity. The evolution of microorganisms: molecular analysis and phylogenetic studies. Eukaryotic micro-organisms

Master's degree in Industrial Bacteriology

BACTERIOLOGY AND MICROBIAL BIOTECHNOLOGY (6 CC)

Microbe/man interactions and possible outcomes. bacterial pathogens: pathogenic power and virulence.
Host defenses: innate and adaptive immunity; bacterial interference: adhesion, colonization virulence factors;
Virulence strategies (toxinogenesis, invasiveness, bacterial evasion from the host defenses; facultative and obligate intracellular life ).
The Human-associated microbiota: composition and variability.
Interaction with the host, influence of age, diet and genetic background; possible outcomes of microbiome expression and microbiota alterations.
Fighting the microbes: antibiotics (classes, mechanisms); bacterial resistance strategies and related problems; The possible strategies to face the “superbugs”: the new vaccines; the phage therapy
Microorganisms and biotechnology: basics, history, research fields. Choosing the optimal host Codon adaptation index and PLS modeling
Industrial employing of microorganisms. Microbes for biotechnologies: taxonomy, features, cultivation, genetic manipulation and heterologous expression in Bacteria: (Escherichia coli; Bacillus, Lactic Acid bacilli –LABs-and Streptomyces) and -Yeasts (Saccharomyces, Hansenula, Pichia, Kluyveromyces)

Master's Degree in Evolutionary Biology and Ecology

ENVIROMENTAL MICROBIOLOGY (6 CC)

Microbial evolution; functional groups. Energy production and environment adaptation mechanisms. Spatial organization of microbial communities: biofilm and mats. Abiotic factors (temperature, water availability, atmosphere, pH, redox value, light..). Extreme environments.
Microbial interactions; Quorum sensing, Microbes and stress. Microbes and biogeochemical cycles (carbon, nitrogen, sulfur, phosphorus, manganese, mercury and minor elements). Bio-leaching. Aqueous and terrestrial environments; microbes-eukaryotes interactions in these contexts . The air and its microbiological control. Microbes and pollutants; treatment of wastewaters and composting. Water-transmitted diseases; potabilization and water quality controls. Bioremediation. Monuments deterioration. Traditional and molecular techniques in the environmental microbiology and microbial ecology.

Additional Courses

Human pathogenic bacteria (BACTERIOLOGY II) (3 CC)

Description, aetiopathogenesis, virulence factors and strategies; identification and control of pathogenic species within the following genera:
Bacillus; Clostridium; Listeria; Staphylococcus; Streptococcus-Enterococcus; Mycoplasma; Corynebacterium; Mycobacterium; Spirochaeta; Chlamydiaceae; Campylobacter-Helicobacter; Brucella; Rickettsiaceae; Neisseria; Bordetella; Haemophilus; Legionella; Enterobacteriaceae: Salmonella, Shigella, Yersinia, E. coli virotypes; Vibrio; Pseudomonas.
Basics about:
Bartonella, Coxiella, Francisella, Anaplasma, Ehrlichia, Burkholderia, Acinetobacter, Moraxella.

Bacteriology-I (3CC)

Microbe/man interactions and possible outcomes. bacterial pathogens: pathogenic power and virulence.
Host defenses: innate and adaptive immunity; bacterial interference: adhesion, colonization virulence factors;
Virulence strategies (toxinogenesis, invasiveness, bacterial evasion from the host defenses; facultative and obligate intracellular life ).
The Human-associated microbiota: composition and variability.
Interaction with the host, influence of age, diet and genetic background; possible outcomes of microbiome expression and microbiota alterations.
Fighting the microbes: antibiotics (classes, mechanisms); bacterial resistance strategies and related problems; The possible strategies to face the “superbugs”: the new vaccines; the phage therapy.

Microorganisms and heterologous expression (3 CC)

Microorganisms and biotechnology: basics, history, research fields. Choosing the optimal host: Codon adaptation index and PLS modeling
Industrially employed microorganisms. Microbes for biotechnologies: taxonomy, features, cultivation, genetic manipulation and heterologous expression in Bacteria: (Escherichia coli; Bacillus, Lactic Acid bacilli –LABs-and Streptomyces) and -Yeasts (Saccharomyces, Hansenula, Pichia, Kluyveromyces).