M.C. Thaller

Bachelor in Biotechnology (8 CC)

General Microbiology and Virology

MODULE I General Microbiology
Historical notes: the evolution of techniques and ideas.
Microbiological methods: cultivation, identification, taxonomy.
Cellular and molecular organization of prokaryotic and eukaryotic cells . The cell wall. The cytoplasmic membrane, transport and secretion systems. Cytoplasmic inclusions, outer envelopes. The organization and structure of the genome. Bacterial metabolism. Anabolism and catabolism; nutritional types and energy scavenging strategies: aerobic and anaerobic respiration, fermentation, oxygenic and anoxygenic photosynthesis. The environmental impact of microbial metabolism: basic knowledge about carbon and nitrogen cycles.
Microbial Growth: nutritional requirements, the struggle for food, nutrient transport and motility. Cell and population growth: Growth curve, abiotic factors, chemo- and other taxis. Sessile growth: the biofilms.
Bacteriophages.
Eucaryotics microorganisms: yeasts, molds and protozoans Genetics and genic expression regulations. Cell division, nucleoid replication and septum formation. Cell-cycle models. Mutations, lateral genetic transfer.Mobile DNA. Homologous and non- Homologous genetic recombination. Gene expression and regulation; Environment and microorganisms: microbes and biogeochemical cycles (carbon and nitrogen cycles), waste-waters treatment and compost production. Micro-organisms and other living beings: interactions between microorganisms, and microorganisms/Eucarya. Parasite/host interactions, virulence Antimicrobial strategies: sterilization, disinfection, antibiotics, action and resistance.
Basics of Immunology: Humoral and cell-mediated immunity. Innate and acquired immunity. The evolution of microorganisms: molecular analysis and phylogenetic studies. Eukaryotic micro-organisms.