A new species of Sphingomonas of biotechnological interest

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Sphingomonads gained particular attention for their ability to degrade a variety of compounds. MCT13 strain was isolated from a water sample collected in September 2007 at the artificial pond within the Laboratory of Experimental Ecology and Acquaculture (University of Tor Vergata, Rome, Italy). The isolate has been assigned to the Sphingomonas genus with the 16S rDNA sequencing. The morphological, biochemical and chemotaxonomic analyses revealed that MCT13 strain represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp. nov. was proposed. This isolate was able to produce evident pitting on Zobell agar plates, suggesting its ability to produce agarases, as was also confirmed by the analysis of MCT13 draft genome, deposited at DDBJ/EMBL/GenBank databases and announced on Frontiers in Environmental Science (2017). Up to now, agarolytic activity has been more often found in marine-bacteria and has not been observed in any of the characterized or validly published Sphingomonas species. The results of preliminary bioinformatic analyses suggest the presence of genes which could be potentially used either in bioremediation or in industrial applications, when the degradation complex carbohydrates is necessary.

 

Thaller M.C., D’Andrea M.M., Marmo P., Civitareale C., Casu F., Migliore L. (2018) - SPHINGOMONAS TURRISVIRGATAE SP. NOV., AN AGAR-DEGRADING SPECIES ISOLATED FROM FRESHWATER. International Journal of Systematic and Evolutionary Microbiology, in press.

 A yellow pigmented and agar-pitting colony was isolated from the water of a drainage ditch within a disused system of constructed wetlands. The strain was purified and named MCT13T. This rod-shaped, Gram-negative, oxidase- and catalase-positive, aerobic, non-spore-forming and non-motile strain formed round colonies and grew optimally at pH 7.0±0.2, at 28–30 °C on LB agar, with 0-0.5% NaCl. The 16S rRNA gene sequence analysis placed the MCT13T isolate within the Sphingomonas (sensu stricto) cluster. The DNA G+C content was 65.3%. The only observed ubiquinone was Q10. The major fatty acids included C17 : 1ω6c and summed feature 8. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major polyamine was spermidine. The 16S rRNA phylogenetic analysis showed the closest relative of MCT13T to be Sphingomonas koreensis (98.48%); however, there are several genotypical and phenotypical differences between the novel isolate and the type strain JSS26T of S. koreeensis. On the basis of these results, the MCT13T strain represents a novel species in the genus Sphingomonas, for which the name Sphingomonas turrisvirgatae sp. nov. is proposed. The type strain is MCT13T (=DSMZ105457T =BAC RE RSCIC7T).

 

D'Andrea M., Ciacci N., Di Pilato V., Rossolini G.M., Thaller M.C. (2017). DRAFT GENOME SEQUENCE OF THE AGARASE-PRODUCING SPHINGOMONAS SP. MCT13. Frontiers in Environmental Science 5:9. DOI: 10.3389/fenvs.2017.00009